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anti cd33  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd33
    Anti Cd33, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd33/CD33+Antibody%2C+anti-human/pm42869916-282-14-19
    Average 94 stars, based on 90 article reviews
    anti cd33 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Interaction between NSCLC Cells, CD8 + T-Cells and Immune Checkpoint Inhibitors Potentiates Coagulation and Promotes Metabolic Remodeling-New Cues on CAT-VTE.
    Article Snippet: .. After 6 days of induction, derived MDSCs were magnetically sorted to CD33+ and CD11b+ cells using the following kits: CD33 MicroBeads—human (130-045-501, Miltenyi Biotec) and CD11b MicroBeads—human (130-049-601, Miltenyi Biotec), according to the manufacturer’s indications. .. Human adenocarcinoma cell line A549 (CCL-185TM, ATCC) and mucoepidermoid carcinoma cell line H292 (CRL-1848TM, ATCC) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Isolation:

    Article Title: Drivers of clinical resistance to venetoclax and hypomethylating agents in acute myeloid leukemia and strategies for improving efficacy
    Article Snippet: .. Blasts were isolated from AML patient samples using CD34 (StemCell Technologies, #17856), CD117 (Miltenyi Biotec, #130‐091‐332), or CD33 (Miltenyi Biotec, #130‐045‐501) immunomagnetic beads. ..

    Article Title: Drivers of clinical resistance to venetoclax and hypomethylating agents in acute myeloid leukemia and strategies for improving efficacy.
    Article Snippet: .. Blasts were isolated from AML patient samples using CD34 (StemCell Technologies, #17856), CD117 (Miltenyi Biotec, #130‐091‐332), or CD33 (Miltenyi Biotec, #130‐045‐501) immunomagnetic beads. ..

    Flow Cytometry:

    Article Title: Control of acute myeloid leukemia by a trifunctional NKp46-CD16a-NK cell engager targeting CD123.
    Article Snippet: For NK cell depletion, 100 μl of polyclonal anti-asialo-GM1 (Poly21460, Biolegend) antibody was injected intraperioneally into recipient mice at the indicated time points. .. A qualified flow cytometry panel composed of antibodies against the antigens CD45 (BD Biosciences ref. no. 563530, Clone D058-1283), CD14 (Miltenyi Biotec ref. no. 130-110-518, clone REA599), CD203c (Invitrogen ref. no. 17–2039, clone NP4D6), CD193 (Biolegend ref. no. 310708, clone 5E8), IgE (Miltenyi Biotec ref. no. 130-117-931, clone REA1049), CD123 (BD Biosciences ref. no. 554529, clone 7G3), CD33 (Miltenyi Biotec ref. no. 130-113-350, clone AC104.3E3) and the viability marker Zombie Nir (Biolegend ref. no. 423106) was used to evaluate the phenotype and Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01626-2 counts of basophils and total CD123-positive immune cells in cynomolgus monkey blood and bone marrow samples. .. Blood (100 μl) and bone marrow samples (50 μl) were collected into a K3-EDTA anticoagulation air-vacuum tubes, incubated with a lysis solution (Biocytex CP025) for 10 min and centrifuged at 300g at room temperature for 5 min with Dulbecco’s PBS (Sigma D8537) before staining for 10 min, washing and fixation.

    Article Title: Delivery of Mycobacterium tuberculosis epitopes by Bordetella pertussis adenylate cyclase toxoid expands HLA-E-restricted cytotoxic CD8 + T cells
    Article Snippet: CD8 + T cells were sorted by using magnetic beads (Miltenyi Biotec) and were co-cultured with unpulsed or Mtb peptide-pulsed THP-1 cells, at an effector:target (E:T) ratio of 20:1 for 6 hrs. .. After incubation, the cells were stained with CD33 (APC, Miltenyi Biotec, clone REA775) used as threshold and Annexin-V-FLUOS staining kit to assess the cytotoxicity of target cells by Flow cytometry ( ). ..

    Marker:

    Article Title: Control of acute myeloid leukemia by a trifunctional NKp46-CD16a-NK cell engager targeting CD123.
    Article Snippet: For NK cell depletion, 100 μl of polyclonal anti-asialo-GM1 (Poly21460, Biolegend) antibody was injected intraperioneally into recipient mice at the indicated time points. .. A qualified flow cytometry panel composed of antibodies against the antigens CD45 (BD Biosciences ref. no. 563530, Clone D058-1283), CD14 (Miltenyi Biotec ref. no. 130-110-518, clone REA599), CD203c (Invitrogen ref. no. 17–2039, clone NP4D6), CD193 (Biolegend ref. no. 310708, clone 5E8), IgE (Miltenyi Biotec ref. no. 130-117-931, clone REA1049), CD123 (BD Biosciences ref. no. 554529, clone 7G3), CD33 (Miltenyi Biotec ref. no. 130-113-350, clone AC104.3E3) and the viability marker Zombie Nir (Biolegend ref. no. 423106) was used to evaluate the phenotype and Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01626-2 counts of basophils and total CD123-positive immune cells in cynomolgus monkey blood and bone marrow samples. .. Blood (100 μl) and bone marrow samples (50 μl) were collected into a K3-EDTA anticoagulation air-vacuum tubes, incubated with a lysis solution (Biocytex CP025) for 10 min and centrifuged at 300g at room temperature for 5 min with Dulbecco’s PBS (Sigma D8537) before staining for 10 min, washing and fixation.

    Article Title: CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition
    Article Snippet: .. For analysis of cell surface marker expression, allophycocyanin (APC)-, phycoerythrin/cyanine 7 (PE/Cy7)-, or PE-Vio770-conjugated anti-human HLA-DR, CD3, CD10, CD11b, CD11c, CD13, CD14, CD15, CD18, CD19, CD27, CD33, CD34, CD41a, CD49a, CD117, and CD135 antibodies were used (BioLegend, San Diego, CA; BD Life Sciences, Franklin Lakes, NJ; or Miltenyi Biotech, Bergisch Gladbach, Germany). .. Isotype controls mouse IgG1, mouse IgG2a, mouse IgM, and recombinant human IgG1 were used accordingly.

    Article Title: CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition
    Article Snippet: .. For analysis of cell surface marker expression, allophycocyanin (APC)-, phycoerythrin/cyanine 7 (PE/Cy7)-, or PE-Vio770conjugated anti-human HLA-DR, CD3, CD10, CD11b, CD11c, CD13, CD14, CD15, CD18, CD19, CD27, CD33, CD34, CD41a, CD49a, CD117, and CD135 antibodies were used (BioLegend, San Diego, CA; BD Life Sciences, Franklin Lakes, NJ; or Miltenyi Biotech, Bergisch Gladbach, Germany). ..

    Incubation:

    Article Title: Delivery of Mycobacterium tuberculosis epitopes by Bordetella pertussis adenylate cyclase toxoid expands HLA-E-restricted cytotoxic CD8 + T cells
    Article Snippet: CD8 + T cells were sorted by using magnetic beads (Miltenyi Biotec) and were co-cultured with unpulsed or Mtb peptide-pulsed THP-1 cells, at an effector:target (E:T) ratio of 20:1 for 6 hrs. .. After incubation, the cells were stained with CD33 (APC, Miltenyi Biotec, clone REA775) used as threshold and Annexin-V-FLUOS staining kit to assess the cytotoxicity of target cells by Flow cytometry ( ). ..

    Staining:

    Article Title: Delivery of Mycobacterium tuberculosis epitopes by Bordetella pertussis adenylate cyclase toxoid expands HLA-E-restricted cytotoxic CD8 + T cells
    Article Snippet: CD8 + T cells were sorted by using magnetic beads (Miltenyi Biotec) and were co-cultured with unpulsed or Mtb peptide-pulsed THP-1 cells, at an effector:target (E:T) ratio of 20:1 for 6 hrs. .. After incubation, the cells were stained with CD33 (APC, Miltenyi Biotec, clone REA775) used as threshold and Annexin-V-FLUOS staining kit to assess the cytotoxicity of target cells by Flow cytometry ( ). ..

    Analysis:

    Article Title: CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition
    Article Snippet: .. For analysis of cell surface marker expression, allophycocyanin (APC)-, phycoerythrin/cyanine 7 (PE/Cy7)-, or PE-Vio770-conjugated anti-human HLA-DR, CD3, CD10, CD11b, CD11c, CD13, CD14, CD15, CD18, CD19, CD27, CD33, CD34, CD41a, CD49a, CD117, and CD135 antibodies were used (BioLegend, San Diego, CA; BD Life Sciences, Franklin Lakes, NJ; or Miltenyi Biotech, Bergisch Gladbach, Germany). .. Isotype controls mouse IgG1, mouse IgG2a, mouse IgM, and recombinant human IgG1 were used accordingly.

    Expressing:

    Article Title: CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition
    Article Snippet: .. For analysis of cell surface marker expression, allophycocyanin (APC)-, phycoerythrin/cyanine 7 (PE/Cy7)-, or PE-Vio770-conjugated anti-human HLA-DR, CD3, CD10, CD11b, CD11c, CD13, CD14, CD15, CD18, CD19, CD27, CD33, CD34, CD41a, CD49a, CD117, and CD135 antibodies were used (BioLegend, San Diego, CA; BD Life Sciences, Franklin Lakes, NJ; or Miltenyi Biotech, Bergisch Gladbach, Germany). .. Isotype controls mouse IgG1, mouse IgG2a, mouse IgM, and recombinant human IgG1 were used accordingly.

    Article Title: CSF3R T618I Collaborates With RUNX1-RUNX1T1 to Expand Hematopoietic Progenitors and Sensitizes to GLI Inhibition
    Article Snippet: .. For analysis of cell surface marker expression, allophycocyanin (APC)-, phycoerythrin/cyanine 7 (PE/Cy7)-, or PE-Vio770conjugated anti-human HLA-DR, CD3, CD10, CD11b, CD11c, CD13, CD14, CD15, CD18, CD19, CD27, CD33, CD34, CD41a, CD49a, CD117, and CD135 antibodies were used (BioLegend, San Diego, CA; BD Life Sciences, Franklin Lakes, NJ; or Miltenyi Biotech, Bergisch Gladbach, Germany). ..

    Purification:

    Article Title: CLUH functions as a negative regulator of inflammation in human macrophages and determines ulcerative colitis pathogenesis
    Article Snippet: .. Monocytes were purified from human peripheral blood mononuclear cells by positive CD33 ( ) selection (Miltenyi Biotec) and cultured with 10 ng/mL macrophage colony-stimulating factor (GenScript) for 7 days as before ( ). ..

    Selection:

    Article Title: CLUH functions as a negative regulator of inflammation in human macrophages and determines ulcerative colitis pathogenesis
    Article Snippet: .. Monocytes were purified from human peripheral blood mononuclear cells by positive CD33 ( ) selection (Miltenyi Biotec) and cultured with 10 ng/mL macrophage colony-stimulating factor (GenScript) for 7 days as before ( ). ..

    Cell Culture:

    Article Title: CLUH functions as a negative regulator of inflammation in human macrophages and determines ulcerative colitis pathogenesis
    Article Snippet: .. Monocytes were purified from human peripheral blood mononuclear cells by positive CD33 ( ) selection (Miltenyi Biotec) and cultured with 10 ng/mL macrophage colony-stimulating factor (GenScript) for 7 days as before ( ). ..



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